Creative Proteomics is the
proteomics division of CD Inc, an integrated CRO company that provides a full
range of drug development services, including Molecular Biology, Biochemistry,
Systems Biology, Organic Chemistry, Genomics, Bioinformatics, Structural
Biology, Preclinical and Clinical studies.
Creative Proteomics offers iTRAQ proteinquantification analysis service suited for unbiased untargeted
biomarker discovery. Relative quantification of proteins for biomarker
discovery in complex mixtures by mass spectrometry can easily and quickly be
achieved using iTRAQ technology. iTRAQ is ideally suited for comparing normal,
diseased, and drug-treated samples, time course studies, biological replicates and
provides relative quantitation.
SILAC-basedquantitative proteomics (SILAQ) is an innovative technology used in high throughput
quantitative analysis of large protein complexes, protein-protein and
protein-small molecule interactions. SILAC service of Creative Proteomics
provides an unbiased strategy that can reveal how specifically either
inhibitors, or other perturbations, affect the dynamic properties and cellular
distributions of proteins. It can also be used as a sensitive and effective
method to determine the specific interaction partners of proteins in the cell.
Di-Sulfide Bond Localization
For many proteins and
peptides, disulfide bridges are prerequisite for their proper biological
function. Many commercialized proteins are cross-linked by disulfide bridges
that increase their resistance to destructive effects of extreme environment
used in industrial processes or protect protein-based therapeutics from rapid
proteolytic degradation. Manufacturing of these products must take into account
oxidative refolding—a formation of native disulfide bonds by specific pairs of
cysteines located throughout a sequence of linear protein.
Disulfidebonds play
an important role in the folding and stability of some proteins, usually
proteins secreted to the extracellular medium. Since most cellular compartments
are reducing environments, in general, disulfide bonds are unstable in the
cytosol, with some exceptions as noted below, unless a sulfhydryl oxidase is
present
